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- digraph g {
- rankdir=LR;
- node [shape=rpromoter colorscheme=rdbu5 color=1 style=filled fontcolor=3]; Hef1a; TRE; UAS; Hef1aLacOid;
- Hef1aLacOid [label="Hef1a-LacOid"];
- node [shape=rarrow colorscheme=rdbu5 color=5 style=filled fontcolor=3]; Gal4VP16; LacI; rtTA3; DeltamCherry;
- Gal4VP16 [label="Gal4-VP16"];
- product [shape=oval style=filled colorscheme=rdbu5 color=2 label=""];
- repression [shape=oval label="LacI repression" fontcolor=black style=dotted];
- node [shape=oval style=filled colorscheme=rdbu5 color=4 fontcolor=5];
- combination [label="rtTA3 + Doxycycline"];
- LacIprotein [label="LacI"];
- rtTA3protein [label="rtTA3"];
- Gal4VP16protein [label="Gal4-VP16"];
-
- subgraph cluster_0 {
- colorscheme=rdbu5;
- color=3;
- node [colorscheme=rdbu5 fontcolor=3];
- Hef1a -> Gal4VP16 [arrowhead=none];
- Gal4VP16 -> UAS [arrowhead=none];
- UAS -> LacI [arrowhead=none];
- LacI -> Hef1aLacOid [arrowhead=none];
- Hef1aLacOid -> rtTA3 [arrowhead=none];
- rtTA3 -> TRE [arrowhead=none];
- TRE -> DeltamCherry [arrowhead=none]
- }
-
- Gal4VP16 -> Gal4VP16protein;
- Gal4VP16protein -> UAS;
- LacI -> LacIprotein;
- LacIprotein -> repression;
- repression -> Hef1aLacOid [arrowhead=tee];
- IPTG -> repression [arrowhead=tee];
- rtTA3 -> rtTA3protein;
- rtTA3protein -> combination;
- combination -> TRE;
- Doxycycline -> combination;
- DeltamCherry -> product;
-
-
-
- }
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